Associations between ISSR Markers and Quantitative Traits in Bread Wheat Genotypes
Galal A. R. El-Sherbeny
Department of Genetics, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt.
Mohamed K. Omara
Department of Genetics, Faculty of Agriculture, Assiut University, Assuit, Egypt.
Ashraf A. Farrage
Department of Genetics, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt.
Abdelsabour G. A. Khaled *
Department of Genetics, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Morphological traits and molecular markers analyses are very important tools for estimation of genetic variability among genotypes. In this study, we combined the phenotypic characterization with the inter-simple sequence repeats (ISSR) technique for the assessment of genetic diversity among 26 bread wheat genotypes. Out of 87 amplified bands 43 were polymorphic. The %P ranged from 25 to 76.92 with an average of 46.97%. The average number of polymorphic bands was 4.30 per primer. UBC-849 and UBC-833 primers which belonged to (GT) and (GA) repeats primer groups produced 62.50 and 76.92% level of polymorphism, respectively. It is clear that the highest polymorphism level was obtained in the case of di-nucleotide repeat primers. In this work the polymorphism information content (PIC) values varied from 0.04 (UBC-819) to 0.19 (UBC-834) with an average of 0.13. The Marker index (MI) values were from 0.12 (UBC-819) to 1.50 (UBC-833) with an average of 0.62. Two markers (UBC-811930bp and UBC-833600bp) were identified which may be linked to stem diameter and grain yield per plant traits, respectively.
Keywords: Phytogeography, Triticum aestivum L., Distribution of Euphorbiaceae, molecular markers, grain yield, polymorphism