Light-Driven Permeability of Chloride Pump
Jin-Won Park *
Department of Chemical and Biomolecular Engineering, College of Energy and Biotechnology, Seoul National University of Science and Technology, 232 Gongneung-Ro, Nowon-Gu, Seoul, 01811, Republic of Korea.
*Author to whom correspondence should be addressed.
Abstract
The effect of light intensity on the permeability of chloride pump was investigated because chloride transport is an essential process in microbes such as halobacteria. The chloride pump was reconstituted into liposomes prepared with dioleoylphosphatidylcholine. The permeability of the liposome layer was estimated by measuring the fluorescence intensity (420 nm) of the 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ) encapsulated in the liposome. The SPQ intensity increased by exposing the liposomes to the 320 nm light, after the chloride was added into the outside of the liposomes. The results of this study seem to be useful in deriving the drug candidate for the pump-relevant disease.
Keywords: Chloride, pump, permeability, liposome